摘要
用IBDV抗体与某IBD商品疫苗制成IBDV-Ab复合苗.用IBDV-Ab复合苗与IBD商品疫苗在有母源抗体的雏鸡中进行免疫对比试验.试验鸡随机分为4组,饲养在隔离器中 第1组为空白对照组,第2组为攻毒对照组,第3组在 10 d龄用复合苗进行免疫,第 4组在 10 d龄用商品苗进行免疫.各组从 11d龄起每隔 10 d采血,用酶联免疫吸附试验检测血清中 IBD抗体水平,35 d龄时进行攻毒试验.试验结果显示,21d龄时,第3、4组的抗体水平分别为738、946;31d龄时,分别为 2 403、1700攻毒后,攻毒对照组死亡率为 50%,第 3、4组分别为 5%、10%.结果表明,在有母源抗体水平的雏鸡中,10 d龄用 IBDV-Ab复合苗免疫的效果优于商品疫苗,结果提示,IBDV高免抗体和中等毒力的IBDV疫苗接一定比例配合后。
The efficacy of a novel vaccine, infectious bursal disease virus-antibody complex vaccine (IBDV-Ab), was compared with that of a commercial IBDV vaccine in Shiqiza broiler with maternal antibodies against IBDV. Seventy-five birds were divided into four groups and raised in isolation units. Birds of group 3 and 4 were vaccinated at 10 days with IBDV-Ab vaccine and commercial vaccine, respectively, whereas chickens of group 1 and 2 were not vaccinated with any vaccine. The antibodies against IBDV were measured by enzyme-linked immunosorbent assay (ELISA) before and after vaccinations. All the broilers of groups 2, 3, 4 were challenged with a virulent IBDV strain at 35 days of age. The mean ELISA titres (ET) of group 3 and 4 at 21 days of age were 738 and 946, respectively, and these at 31 days of age were 2 403 and 1 700. After challenge, mortality of groups 2, 3 and 4 were 50% 1 5% and 10% , respectively. The results showed that the efficacy of vaccination with IBDV-Ab vaccine was better than that with commercial vaccine. The data herein suggested that being mixed with antibody against IBDV increase the efficacy of IBDV live vaccine.
出处
《华南农业大学学报》
CAS
CSCD
北大核心
2000年第1期75-77,共3页
Journal of South China Agricultural University
关键词
传染性法氏囊病
免疫
鸡
抗原抗体复合苗
疫苗
infectious bursal disease
IBDV-antibody complex vaccine
vaccination