姜黄素对HepG2细胞蛋白质组的影响

Effect of curcumin on proteome of HepG2 cell

目的:应用双向电泳和质谱技术研究姜黄素对HepG2细胞蛋白质组的影响。方法:双向电泳分离HepG2细胞和经过30μmol/L姜黄素处理的HepG2细胞蛋白质,经银染后扫描得到蛋白质组图谱,用Image Master2D 6.0软件进行差异蛋白质组分析,筛选差异在2倍以上的蛋白质作为差异蛋白,进行质谱鉴定,并用RT-PCR方法进行初步验证。结果:经姜黄素处理后,HepG2细胞中蛋白质二硫键异构酶、复制蛋白A2、肉碱缺乏症相关蛋白、细胞周期蛋白依赖性激酶抑制因子表达量增加,而增殖细胞核抗原、内质网60蛋白酶、Ran结合蛋白1、Stathmin 1/oncoprotein 18、Nm23蛋白表达量降低。结论:姜黄素可以通过干扰细胞周期变化、抑制肿瘤细胞的生长、促进细胞凋亡、抑制肿瘤浸润与转移及促进细胞损伤修复而起到抗肿瘤作用,此外,姜黄素降血脂和抗动脉粥样硬化作用可能与促进脂肪酸跨膜转运、维护细胞膜稳定性相关。

Objective： Two-dimensional electrophoresis and mass spectrum were used to study the effect of curcumin on the proteome of HepG2 cell.Methods： The proteins from HepG2 cell and HepG2 cell treated with 30μmol/L curcumin were separated with two-dimensional electrophoresis and the gels were scan after silver staining.All images were analyzed by Image Master 6.0 software,and the different proteins were identified by mass spectrum.Result： The expression of protein disulfide isomerase,replication protein A2,carnitine deficiency-associated gene and cyclin-dependent kinase inhibitor 2A increased in the HepG2 cell treated with 30μmol/L curcumin,while the expression of PCNA,ER-60 protease,RNA binding protein 1,Stathmin 1/ oncoprotein 18,Nm23 protein decreased.Conclusion： Curcumin could inhibit tumor cell growth,interfere cell cycle,promote apoptosis,inhibit tumor invasion and metastasis,and promote cell repair.In addition,the hypolipidemic and anti-atherosclerosis effects of curcumin may correlate with promotion of the transmembrane transport of fatty acids and maintain of cell membrane stability.