植物性食品中氯丹残留量快速测定方法

Rapid Determination Method of Chloramphenicol Residues in Aquaculture Water

建立用气相色谱仪法快速测定植物性食品中氯丹残留量。试样经丙酮提取,正己烷萃取,使用HP-5色谱柱分离,ECD测定。结果表明,顺式氯丹、反式氯丹与氧氯丹能够很好分离,在0.2～100μg/L线性关系良好(r≥0.999)。在2.0～10.0μg/kg的添加水平范围内的平均回收率为82.8%～94.6%,相对标准偏差为2.0%～6.5%。顺式氯丹、反式氯丹与氧氯丹该方法检出限均为0.5μg/kg。

A high liquid chromatography coupled with eletrospray ionization quadrupole tandem mass spectrum etry rapid determination method of chloramphenicol residues in aquaculture water. An ultra Performance liquid chromatography coupled with eletrospray ionization quadrupole tandem mass spectrum etry was used. The residue was quantified with multiple reactiong monitoring （MRM） mode. The method was validated and good results were obtained with respect to precision, repeatability and spiked recovery. The limit of detection was 0.005 p-g/L for chloramphenicol, and the linear range was from 0.2 to 100 μg/L. The average recoveries were between 82.8 % to 94.6 % in the spiked range of 0.05-0.5 μg/L, and the relative standard deviations（RSD） were between 2.0 % to 6.5 %. The method has good repeatability and high sensitivity （r=0.999）, and can be applied for the determination of chloramphenicol residue in aquaculture water.