基于ITS2条形码的五加皮基原植物及其易混品的鉴定

Molecular Identification of Acanthopanacis Cortex Original Plant and Its Adulterants Based on ITS2 DNA Barcode

目的:对五加皮基原植物及其易混品进行分子鉴别,为临床用药提供科学依据。方法:从GenBank数据库下载五加皮基原植物及其易混品的ITS2序列,经CodonCode Aligner,MEGA4.0等软件进行序列拼接和分析,构建NJ(邻接)、ME(最小进化)树,并应用Koetschan等建立的ITS2数据库及其网站预测ITS2二级结构辅助分析。结果:基于ITS2序列的两种系统聚类树都易于将五加皮基原植物细柱五加与其易混品鉴别开;比较与细柱五加亲缘关系较近的同属易混品二级结构可以看出,细柱五加在螺旋区茎环的数目、大小、位置以及螺旋臂由中心环伸出时的转角等方面,与其易混品间具有较明显区别。结论:ITS2序列可以将五加皮基原植物及其易混品区分开,在药用植物基原鉴定方面具有重要应用价值。

Objective ：To discriminate Acanthopanacis cortex original plant and its adulterants, and assure me authenticity and clinical curative effect of this medical material. Methods： Download the ITS2 barcode sequences of Acanthopanax gracilistylus and its adulterants from generation were performed using the CodonCode GenBank database. Sequence assembly and consensus sequence Aligne r. Sequence analyses were carried out by MEGA4. Phylogenetic