肠道病毒71型和柯萨奇病毒A16型抗体筛查在手足口病中的应用及相关性

Application and correlation of enterovirus 71 and Coxsackie virus A16 antibody screening in the hand, foot and mouth disease

目的探讨肠道病毒71型（EV71）和柯萨奇病毒A16型（CA16）抗体筛查在手足口病中的应用及相关性。方法采用酶联免疫吸附法对确诊的281例手足口病患儿进行血清EV71-IgM抗体和CA16-IgM抗体检测。结果EV71．IgM抗体阳性144例（51．2％，144／281），CA16-IgM抗体阳性90例（32．0％，90／281），EV71-IgM和CA16-IgM双阳性17例（6．0％，17／281），即抗体阳性确切例数为217例，抗体阳性率为77．2％（217／281）。EV71-IgM抗体阳性率与CA16-IgM抗体阳性率比较差异有统计学意义（r=21．35，P〈0．01），总体男性与女性抗体阳性率[95．1％（135／142）比71．2％（99／139）]比较差异有统计学意义（χ^2=8．22，P〈0．01）。结论早期进行EV71-IgM和CA16-IgM抗体联合检测，对临床诊断手足口病及监控治疗具有重要意义。

Objective To investigate the application and correlation of enterovirus 71 （EV71） and Coxsackie virus A16 （CA16） antibody screening in the hand, foot and mouth disease （HFMD）. Methods The method of enzyme linked immunosorbent assay was used to test the serum antibody of EV71-IgM and CA16-IgM in 281 patients （ 142 male and 139 female） with HFMD. Results The positive rate of EV71-IgM antibody was 51.2% （144/281）, the positive rate of CA16-IgM antibody was 32.0% （90/281）. Seventeen patients（6.0%, 17/281 ） had dual positive of EV71-IgM and CA16-IgM antibody. The total detection rate was 77.2%（217/281 ）. The positive rate of EV71-IgM and CA16-IgM antibody had significant difference （χ^2 = 21.35 ,P 〈 0.01 ）. The positive rate of EV71-IgM and CA16-IgM antibody in male bad significant difference compared with female [95.1%（135/142） vs. 71.2%（99/139） ,P〈 0.01 ]. Conclusions At the beginning of the illness should use the joint detection of EVTI-IgM and CA16-IgM antibody diagnose HFMD. It has significance in diagnosing and monitoring.