高铜日粮对生长猪胃底腺Ghrelin分泌的影响

Effects of high dietary copper on ghrelin levels in the fundus gland of growing pigs

选用军牧1号断乳仔猪45头,随机分为3组,每组5个重复,每个重复3头,采用完全随机化设计进行生长试验,研究了不同来源(硫酸铜和蛋氨酸铜)和不同添加水平(5、125 mg/kg)的高铜日粮对猪胃底腺Ghrelin分泌的影响。结果表明:与对照组相比,日粮中添加125 mg/kg硫酸铜和125 mg/kg蛋氨酸铜组平均日增重、绝对增重、平均采食量均显著提高(P<0.05),而不同铜源间差异不显著(P>0.05);试验组胃底腺Ghrelin mRNA水平显著提高(P<0.05);血液胃泌素含量显著增加(P<0.01),并且Ghrelin mRNA水平与血液胃泌素含量呈显著正相关关系。提示高铜可促进生长猪胃底腺Ghrelin的分泌,胃泌素的分泌与胃底腺区Ghrelin的基因表达有关。

The experiment was conducted to examine the effects of dietary copper supplementation on ghrelin mRNA expression levels in the fundus gland of growing pigs. Forty-five cross-bred pigs were randomly assigned to three groups of 15 pigs, each comprising five replicates of 3 animals. Pigs were allocated to diets that contained 5 mg/kg （.as a control）, 125 mg/kg sulphate copper and 125 mg/kg methionine copper, respectively. At the end of the experiment five pigs were selected at random from each group, slaughtered and collected the fundus gland for determination of ghrelin mRNA expression levels. The results showed that average daily gain, average daily feed intake, absolute weight and ghrelin expression levels were higher in the pigs fed the diets with 125 mg/kg methionine copper and 125 mg/kg sulphate copper （P〈0. 05）, than in the pigs fed the diet with 5 mg/kg copper, Furthermore, gastrin concentration of blood was dramatically higher in the pigs fed the diets with 125 mg/kg methionine copper and 125 mg/kg sulphate copper （P〈0. 01 ） compared to those in 5 mg/kg copper group. The data indicated that high dietary copper appears to increase feed intake and promote weight gain by enhancing ghrelin mRNA expression levels in the fundus gland of pigs. Gastrin secretion might relate to ghrelin gene expression of fundus gland.