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Immobilization of laccase by Cu^(2+) chelate affinity interaction on surface modified magnetic silica particles and its use for the removal of pentachlorophenol 被引量:5

Immobilization of laccase by Cu^(2+) chelate affinity interaction on surface modified magnetic silica particles and its use for the removal of pentachlorophenol
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摘要 Magnetic Cu^(2+)-chelated silica particles using polyacrylamide as a metal-chelating ligand was developed and used for the immobilization of laccase by coordination.The effect of pH and temperature on the enzymatic property of immobilized laccase and its catalytic capacity for pentachlorophenol(PCP) degradation were evaluated systemically.Compared with free laccase,the immobilized laccase showed improved acid adaptabihty and thermal stability.The immobilized laccase prepared in this work exhibited a good catalytic capacity for PCP removal from aqueous solutions. Magnetic Cu^(2+)-chelated silica particles using polyacrylamide as a metal-chelating ligand was developed and used for the immobilization of laccase by coordination.The effect of pH and temperature on the enzymatic property of immobilized laccase and its catalytic capacity for pentachlorophenol(PCP) degradation were evaluated systemically.Compared with free laccase,the immobilized laccase showed improved acid adaptabihty and thermal stability.The immobilized laccase prepared in this work exhibited a good catalytic capacity for PCP removal from aqueous solutions.
出处 《Chinese Chemical Letters》 SCIE CAS CSCD 2012年第2期197-200,共4页 中国化学快报(英文版)
基金 supported by the National Natural Science Foundation of China(Nos21177013 and 50708007) the National Basic Research Program of China(973 Project,No2010CB429003) the Fundamental Research Funds for the Central Universities special fund of State Key Laboratory of Water Environment Simulation(No 11K04ESPCN)
关键词 Magnetic silica Immobilization Laccase Pentachlorophenol Magnetic silica Immobilization Laccase Pentachlorophenol
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