孕哺期双酚A暴露对雄性仔鼠海马即早基因表达的影响

Effects of Exposure to Bisphenol A on the Expression of Immediate Early Genes in the Hippocampus of Male Offspring Rats

目的探讨孕哺期双酚A暴露对雄性仔鼠海马即早基因c-fos、c-jun、egr-1和Arc表达的影响。方法低、中、高剂量双酚A组孕鼠分别灌胃染毒2mg／kg、10mg／kg、50mg／kg双酚A，对照组孕鼠用同体积的玉米油灌胃，至哺乳期结束，检测雄性仔鼠的学习记忆能力以及海马c-los、c-jun、egr-1和Arc水平。结果双酚A组雄性仔鼠在水迷宫中的逃离潜伏期明显高于对照组，海马c-los、c-jun和egr-1水平显著低于对照组，具有一定的剂量一反应关系。结论孕哺期双酚A暴露损害雄性仔鼠学习记忆能力的机制可能与海马c-fos、c-jull和egr-1表达降低有关。

Objective To study the effects of exposure to bisphenol A （BPA） on the expression of immediate early genes including c-fos, c-jun, egr-1 and Arc in the hippocampus of male offspring rats. Methods Low-, middle-, and high- dose BPA groups were administrated with 2, 10, 50 mg/kg BPA, respectively, and control group was given to corn oil of same volume by gavage during prenancy and lactation. Learning, memory and c-fos, c-jun, egr-1 and Arc expression were measured. Results Escape latency of BPA groups in the water maze test were higher significantly than that of control group, and c-fos, c-jun and egr-1 expression levels in the hippocampus of BPA groups were lower significantly than those of control group, presenting dose-effect relationship. Conclusion Exposure to BPA injured learning and memory of male offsprings rats, which was associated with low levels of c-fos, c-jun and egr-1 in the hippocampus.