吡虫啉与蝉拟青霉的相容性及增效作用研究

Compatibility of Imidacloprid and Paecilomyces cicadae and Synergism against Aphids

为了提高蝉拟青霉的杀虫毒力,研究了吡虫啉对蝉拟青霉菌落生长、产孢、孢子萌发的影响及菌药混用的杀虫效果。结果表明,致死剂量(1∶3 000)的吡虫啉对蝉拟青霉菌落生长和产孢有一定的影响,而亚致死剂量(1∶15 000)和次亚致死剂量(1∶30 000)的吡虫啉对菌落生长、产孢、孢子萌发均无显著影响,其对菌落生长的抑菌率分别为2.53%和0.73%,对孢子萌发的抑制率仅为1.02%、-0.35%。该药剂亚致死剂量和次亚致死剂量分别与蝉拟青霉孢子液混用后,对蚜虫的致死中时(LT50)比单用孢子液提前了0.37d、0.26d,蚜虫的校正死亡率也增加。表明亚致死剂量和次亚致死剂量的吡虫啉与蝉拟青霉具有良好的相容性,混用后具有明显的增效作用。

The effects of imidacloprid on colony growth,conidial sporulation and germination of Paecilomyces cicadae were tested.The results showed that the lethal dose（1∶3 000） imidacloprid inhibited mycelium growth and sporulation to a certain degree,but the sublethal dose（1∶15 000）and low sublethal dose（1∶30 000）of imidacloprid had no apparent effect on mycelium growth,conidial sporulation and germination.Their inhibition rates were 2.53% and 0.73% on colony growth,and 1.02% and-0.35% on conidial germination.These indicated that the sublethal dose and low sublethal dose of imidacloprid had compatibility with P.cicadae.The result of a bioassay showed obvious synergism against aphids when P.cicadae was mixed with the sublethal dose and low sublethal dose of imidacloprid,with LT50 0.37 days and 0.26 days earlier and the corrected mortality rate higher than that of P.cicadae alone.