166例ANA阳性者血清线性免疫ANA谱的结果分析

Analysis the Results of Line Immunoassay in 166 Patients with Antinuclear Antibodies Positive

目的了解皖北及沿淮地区血清ANA阳性者中常见自身抗体的阳性率,探讨线性免疫分析法(LIA)对常见结缔组织病的诊断价值。方法用LIA法检测166例患者ANA筛查ELISA检测值大于正常值上限的血清。结果 166例中154例有阳性结果,阳性率为92.8%,139例有2个或以上条带阳性,占阳性结果的90.3%。66例anti-dsDNA抗体阳性;70例抗核小体抗体阳性;37例抗组蛋白抗体阳性;58例anti-SmD1抗体阳性;76例anti-U1snRNP抗体阳性;103例anti-SSA/Ro60抗体阳性;77例anti-SSA/Ro52抗体阳性;46例anti-SSB/La抗体阳性;40例抗P0抗体阳性;10例抗着丝点抗体阳性,抗Scl-70抗体和抗Jo-1抗体抗体各7例阳性。结合临床资料:101例符合SLE;28例符合pSS;14例符合MCTD;7例符合SSc;3例符合UCTD;7例符合PM/DM;6例符合RA。结论皖北及沿淮地区ANA阳性者以SLE最为常见,其次为pSS和MCTD患者;LIA法检测ANA阳性者血清有助于明确结缔组织病的诊断。

Objective The purpose of this .study was to analyze surem antinuclear antibodies positive in the Regions along Huaihe River and the North Anhui province the positive rate of common antibodies, and to explore the diagnosis value of ANA- LIA to the common autoimmune disease. Methods Line immunoassay method was used to detect with 166 patients serum that ANA ELISA analysis quantitative assessment screening greater than normal reference value of upper limit. Results 154 cases of 166 cases had positive results,the positive rate was 92.8%, 139 cases had 2 or more bands of positive,90.3% of the positive re- suits. 66 cases of anti-dsDNA antibody positive;70 cases of anti-nucleosome antibody positive;37 cases of anti-histones antibody positive ;58 cases of anti-StuD1 antibody positive ;76 cases of anti-UlsnRNP antibody positive; 103 cases of anti-SSA/Rot0 anti- body positive ;77 cases of anti-SSA/Ro52 antibody positive ;46 cases of anti-SSB/La antibody positive ;40 cases of anti-P0 anti- body positive;10 cases of anti-centromeres antibody positive;both of anti-Scl70 antibody and anti-Jol antibody have 7 case posi- tive. Combined with clinical data： 101 patients were diagnosed as SLE; 28 patients diagnosed as pSS;14 patients diagnosed as MCTD ;7 patients diagnosed as SSc ;3 patients diagnosed as UCTD ;7 patients diagnosed as PM/DM ;6 patients diagnosed as RA. Conclusion In the Regions along Huaihe River and the North Anhui province, ANA positive was most common in SLE,followed by patients with pSS and MCTD;line immunoassay（ANA-LIA） analysis of ANA-positive serum was helpful to clear the specific diagnosis of connective tissue disease.