海带多糖对铅中毒大鼠生殖系统的影响

Effects of Alginate on Reproductive System of Wistar Rats with Lead Poisoning

通过建立大鼠铅中毒模型,研究海带多糖对铅中毒大鼠生殖系统的影响。大鼠48只,雌雄各半,随机分为正常对照组(正常饮食30d)、模型组(自由饮用0.3%醋酸铅水溶液30d)、青霉胺组(自由饮用0.3%醋酸铅水溶液30d+按90mg.kg-1体重ip青霉胺21d)、海带多糖高、中、低剂量组(自由饮用0.3%醋酸铅水溶液30d+200mg.kg-1、100mg.kg-1,50mg.kg-1ig海带多糖)。实验结束处死大鼠,取血测定血铅,取睾丸、附睾、卵巢、子宫等生殖器官,称重并计算脏器指数,观察雄鼠精子数、死亡精子数、畸形精子数,测定促卵泡成熟激素(FSH)、生殖激素睾酮(T)、促黄体生成激素(LH)的变化。结果表明,海带多糖能降低铅中毒后大鼠的血铅,附睾、睾丸指数降低情况得到改善(P<0.05),精子的畸形率、死亡率有所降低(P<0.05);精子活动率、密度有所增高(P<0.05);T、FSH水平的降低得到改善(P<0.05),而对LH的水平影响不大(P>0.05)。这说明海带多糖对铅中毒大鼠的生殖系统有保护作用,有望开发成天然低毒的铅中毒保护药。

To study the effect of alginate on the reproductive system of the lead poisoning Wistar rats, the following methods was taken： 1） normal control group, After^-1 week＇s exposure to lead, the Wistar rats were randomly divided into 5 groups; 2） model group（0.3%Pb（AC）2）; 3） Penicillamine group（90mg · kg^-1） 4）high-dosealginategroup（200mg· kg^-1）; 5） middle-dosealginategroup（100mg, kg^-1）; 6） lowdose alginate group（50mg · kg-1）. After 1 month＇ treatment, blood lead, genital weight, sperm activity, sperm deformity rate and endocrine function impact were measured. Results show that alginate can lower the blood lead in Wistar rats, improve the epididymis and testis index（P〈0.05）, lower sperm deformity and mortality（P〈0.05）, and improve T, FSH levels（P〈0.05）. The alginate has a protective effect on the reproductive system in Wistar rats with lead poisoning, and is expected to develop into a natural and low toxicity protection medicine to treat lead poisoning.