光谱在生物学研究中的应用

Application of optical spectroscopy in biological research

最近几十年有关光学和振动光谱在生物大分子结构解析理论和应用方面的研究有较快进展.利用光谱、特别是振动光谱来研究生物大分子形貌和动态,如:蛋白质折叠及相关问题是当今生物物理和生物化学领域关注的热点话题.该文介绍光谱包括拉曼、红外光谱在这些领域的应用和发展,结合作者自己研究实际,介绍生物光谱一般的理论,并列举一些实例,探讨生物光谱在研究多肽-蛋白质无序折叠、蛋白酶的功能-结构关系,以及辐射生物学中离子辐射对蛋白质和DNA及组分损伤反应等方面的应用.

Rapid progress has been made in both theories and practice for the application of optical spectroscopy in biology, e.g. , the application of biospectroscopy in the study of biomolecular conformations, kinetics and dynamics, protein folding and etc. , which are the hot topics in the current biophysical and biochemical research fields. This review attempted to introduce some basic theories in biospectroscopy, and also gave some examples related to the author＇s own research activities, and therefore to illustrate the useful application of biospectroscopy in the studies of peptide＇s secondary structure, protein structure - function relationship, and ion-induced effects on proteins and DNA in radiobiology.