摘要
采用溶剂萃取结合GC/MS分析了不同保存期的二次调配烟用香精的香味成分,并运用主成分分析(PCA)法和t检验法分析了香精组分的变化。结果表明:二氯甲烷萃取能最大程度地提取香精的成分,且具有较好的重复性。用PCA和t检验法处理不同保存期烟用香精的GC/MS分析数据,可了解不同存放期香精样品整体变化特征及组分含量的显著性变化情况,为二次调配烟用香精样品的稳定性及存放期提供参考。
The aroma components in secondarily formulated flavor blocks of different storage durations were analyzed by solvent extraction combining with GC/MS.The changes of these components were evaluated by principal component analysis and t-test.The results showed that the aroma components in the samples could be satisfactorily extracted by dichloride methane with good repeatability.The results of GC/MS analytical data processed by principal component analysis and t-test reflected the variation of the samples as a whole and significance of changes of individual aroma component during storage,which provided reference for the evaluation of the stability and storage duration of secondarily formulated flavor blocks.
出处
《烟草科技》
EI
CAS
北大核心
2012年第2期39-42,共4页
Tobacco Science & Technology
基金
中国烟草广东工业有限公司(No.I05XM-QK[2008]017)
国家自然科学基金(No.20875106)
广东省自然科学基金(No.9151027501000003)资助项目
关键词
烟用香精
溶剂萃取
主成分分析
T检验
Tobacco flavor
Solvent extraction
Principal component analysis
t-test Determination of NNN and its Metabolites in Rabbit Blood with HPLC-MS/MS ZHAO BEI-BEI（1）
WANG SHENG（1）
WANG JUAN（2）
WANG ZHAO-YU（3）
and ZHANG JIAN-XUN（1） 1.Zhengzhou Tobacco Research Institute of CNTC
Zhengzhou 450001
China 2.Quality Supervision ＆ Test Station of Hainan Tobacco Corporation
Haikou 571100
China 3.Quality Supervision ＆ Test Station of Zhejiang Tobacco Corporation
Hangzhou 310001
China Abstract： In order to accurately determine N-nitroso nornicotine（NNN） and its metabolites in rabbit blood
a high-performance liquid chromatography/tandem mass spectrometry method was established by optimizing chromatographic conditions and mass spectrometric conditions and using NNN-d4 as an internal standard.The samples were successively pretreated by protein precipitation
separated by a Waters-HILIC column
and analyzed by tandem mass detector under multi-reaction monitoring（MRM） mode.The results showed that the recoveries of NNN and its metabolites ranged from 80% to 111% with the relative standard deviations（RSDs） of 0.5% to 8.62% and the limits of detection of 0.039 to 0.217 ng/mL.This method is simple
sensitive
rapid
and suitable for the analysis of NNN and its metabolites in blood. Keywords： N-nitroso nornicotine（NNN）
Metabolite
HPLC-MS/MS
