大鼠同种异体胎肝组织微片移植治疗慢性肝衰的实验研究

Study of heterografting of microliver slice in rats with cirrhosis of liver

目的探讨大鼠胎肝组织微片移植对慢性肝衰大鼠的治疗作用及可行性,为临床胎肝组织微片移植提供动物实验依据。方法选择肝硬化模型SD大鼠20只,随机分为对照组(n=10)与移植组(n=10)。孕14d的SD胎鼠肝组织作为供体。移植组每只受体接受3个胎鼠肝组织微片移植,移植部位在受体肝组织中;对照组只行开腹手术。手术后1周检测受体ALT、AST、TBIL及ALB的变化;1个月后观察死亡大鼠数量,并处死存活的大鼠,取肝组织检测PCNA、ALB及糖原的表达水平。结果与对照组比较,移植组ALT、AST及TBIL明显下降,ALB显著升高。PCNA、ALB及糖原染色阳性率明显高于对照组。结论大鼠同种异体胎肝组织微片移植可以改善肝硬化大鼠的生存时间及肝功能;移植肝组织可以在受体肝组织内存活,手术方法安全可行。

Objective To study the effect of heterografting of microliver slice in rats with cirrhosis of liver and present the animal,experimental data for clinical trials. Methods 20 Sprague-Dawley rats with cirrhosis of liver were selected as contrast group（n= 10） and transplantation group （n= 10） randomly.Liver tissues removed from E 14 embryos of rats were transplantated to livers of transplantation group.One week after operation,the ALT,AST,TBIL and ALB were detected in two groups.One month later,all alive rats were killed and PCNA,ALB and glycogen were detected by immunohistochemisty stains. Results Compared the control group and the transplantation group, ALT, AST and TBIL decreased greatly and ALB increased obviously in transplantation group; PCNA,TP and glycogen stains increased in transplantation group than that in contrast. Conclusion Heterografting of microliver slice can improve the liver function in rats with cirrhosis of liver,the operation is feasible and safe.