海星脑苷脂-磷脂复合脂质体抗肿瘤活性作用

Antitumor effects of liposome containing cerebroside and phosphatidylcholine separated from Asterias rollestoni

目的通过体内、体外实验,研究海星脑苷脂-磷脂复合脂质体的抗肿瘤作用。方法从罗氏海盘车中提取脑苷脂和磷脂,并制备脑苷脂-磷脂复合单室脂质体,采用四甲基偶氮噻唑蓝(MTT)法测定海星脑苷脂-磷脂复合脂质体对S180细胞生长抑制作用;通过体内实验检测其对S180小鼠实体瘤生长的抑制率、脾脏指数、胸腺指数及体重变化等指标。结果海星脑苷脂-磷脂复合脂质体在体外对S180细胞有明显的抑制作用,其24、487、2 h的半数抑制浓度(IC50)分别为199.89、148.95和126.65μg/mL;体内实验表明,海星脑苷脂-磷脂复合脂质体能明显抑制小鼠S180肉瘤的生长,其抑瘤率为72.6%,同时提高荷瘤小鼠的胸腺指数和脾指数,延缓荷瘤小鼠体重的下降,抑癌效果优于海星脑苷脂和磷脂单独使用。结论海星脑苷脂-磷脂复合脂质体在体内、体外均具有较强的抗肿瘤活性。

Objective To explore antitumor effects of liposomes containing cerebrosides and phosphatidylcholine separated from Asterias rollestoni in vitro and in vivo.Methods Phosphatidylcholine and cerebrosides separated from Asterias rollestoni were used to prepare unilayer liposomes in vitro.3-（4,5 dimethylthiazol-2-yl）2,5 diphenyl tetrazolium bromide（MTT） assay was used to measure the inhibitory effect in S180 cells in vivo.The liposomes were given to the transplanted S180-bearing mice.The tumor weight,spleen index,thymus index,and the changes of body weight of the mice were detected.Results In vitro,the liposomes showed prominent inhibitory effect on the growth of S180 cells with the fifty-percent inhibitory concentration（IC50） of 199.89 μg/mL,148.95 μg/mL,and 126.65 μg/mL,respectively,after 24 hr,48 hr and 72 hr.In vivo,the liposomes obviously inhibited the growth of tumor in S180-bearing mice and the tumor inhibition rate was 72.6%.Furthermore,the thymus index and spleen index increased and the weight loss of S180-bearing mice attenuated in the mice treated with liposomes.Conclusion The liposomes containing cerebrosides and phosphatidylcholine separated from Asterias rollestoni have significant antitumor effect in vitro and in vivo.