高山蓍嫩茎无性系的建立

Establishment of regeneration system for Atractylodes japnica using young stem

【目的】建立高山蓍嫩茎再生繁殖体系,满足高山蓍人工栽培对种苗的需求,保护其野生种质资源。【方法】以嫩茎为外植体,分别探讨不同培养基和激素组合对愈伤组织的诱导、分化、不定芽生根、试管苗继代增殖培养的影响。【结果】在添加不同激素组合的MS、1/2MS、8114和B5培养基中,高山蓍嫩茎愈伤组织诱导培养的理想培养基为1/2MS+6-BA0.8mg/L+NAA0.5mg/L+2,4-D1.5mg/L和1/2MS+6-BA0.8mg/L+NAA0.5mg/L+2,4-D2.0mg/L;愈伤组织分化培养的理想培养基为1/3MS和1/3MS+IAA0.2mg/L;在添加NAA0.6mg/L或IAA0.6mg/L的1/2MS、1/4MS、N6、B5和LS培养基中,1/4MS+IAA0.3mg/L是不定芽生根培养和试管苗继代增殖的理想培养基,生根率为92%,平均每代繁殖系数为2.7;试管苗易移栽定植,成活率可达98.6%。【结论】建立了高山蓍嫩茎繁殖无性系,定植的试管苗生长旺盛,翌年开花结果,并保持了高山蓍的所有植物学性状。

[Objective]In order to protect wild germplasm resources and fulfill seedling demands for artificial cultivation, the present experiment was conducted to develop a regeneration system for Atractylodes japnica using young stem. [Method]The young stem of A. japnica was used as explants to investigate the effects of different culture media and hormone combinations on callus induction and differentiation, rooting of adventitious buds, multiplication of test-tube plantlets and transplantation. [Result]Among different culture media supplemented with different hormone combinations, viz., MS, 1/2MS, 8114 and B5,1/2MS＋6-BA 0.8 mg/L mg/L＋NAA 0.5 mg/L＋2,4-D 1.5 mg/L and 1/2MS＋6-BA 0.8 mg/L＋NAA 0.5 mg/L＋2,4-D 2.0 mg/L were optimum for callus induction, and 1/3MS and 1/3MS＋ IAA 0.2 mg/L were ideal for callus differentiation. Of the 1/2MS, 1/4MS, N6 and B5, LS media supplemented with NAA （0.6 mg/L） and IAA （0.6 mg/L） and 1/4MS with IAA （0.3 mg/L） was optimum for rooting of adventitious buds and subculture multiplication of test-tube plantlets. The rooting rate was recorded as 92% and the average multiplication coefficient was 2.7. The test-tube plantlets were easy to be transplanted and survive, and showed 98.6% survival rate. [Conclusion]The regeneration system for Atractylodes japnica was established and the transplanted plantlets grew vigorously, and flowered and fruited next year with all characteristics of wild plant.