主成分分析法对番茄品种耐弱光性的综合评价

Comprehensive Evaluation of Weak Light Tolerance of Tomato Cultivars with Main Factor Analysis

为了筛选出可用于鉴定番茄耐弱光性的数量化指标,对番茄的耐弱光性进行较系统的综合评价。以6个番茄为试验材料,主要采用分光光度计法对不同程度弱光(50%自然光、25%自然光)胁迫后番茄9个生理生化指标进行测定,利用模糊数学中求隶属函数值的方法对各番茄品种进行耐弱光性综合评价;对番茄各品种抗氧化酶(POD、SOD、CAT)活性、叶绿素含量、丙二醛含量、脯氨酸含量、可溶性蛋白质含量和产量构成因素(坐果率、单株产量)的测定,对各单项指标的耐弱光系数进行相关性分析和主成分分析。试验结果表明,坐果率和产量与其他耐弱光指标之间存在着极显著的相关性。同一种作物在不同程度的逆境胁迫下,抗逆能力的稳定性也有不同。如‘新粉八号’和‘金冠五号’的耐弱光性比较稳定;‘粉都丽人二号’适于在轻度弱光条件的环境中种植;‘亚洲粉皇’对弱光抵御能力最低。

The aim was to screen the quantitative indices of tomato weak light tolerance and synthetically evaluate the weak light tolerance of the various tomato cuhivars. Six tomato cultivars as experimental material were treated by light stress （50% and 25% of normal light density）, then the 9 physiological indices were tested with spectrophotometer, and the weak light tolerance of the various tomato cultivars were evaluated with subordinate function analysis method of fuzzy mathematics. The contents of chlorophyll, MDA, proline and soluble protein, fruit setting percentage and average product per plant were determined, and the correlation analysis and main factor analysis of weak light tolerance coefficient of all the determination indices were analyzed. The results showed that the fruit setting rate and yield had significant correlation with other weak light tolerance indices. The tolerance of ‘Xinfen No.8＇ and ‘Jinguan No.5＇ were most stable for weak light stress. The mild light condition was suitable for ‘Fenduliren No.2＇. ‘Yazhoufenhuang＇ had the worst tolerance for weak light.