皖南地区225例无精子症及严重少精子症患者的细胞遗传学分析

Cytogenetic causation of azoospermia and severe oligospermia in 225 patients from south Anhui areas

目的:研究无精子症及严重少精子症患者与染色体异常之间的关系。方法:对225例无精子症及严重少精子症患者抽取外周血进行淋巴细胞培养、常规G显带、核型分析。结果:共检出染色体异常核型64例,异常率28.44%。其中性染色体异常39例(包括2例性反转),占异常率的60.94%;常染色体结构异常4例,占异常率6.25%;染色体多态性变异21例,占异常率32.81%。结论:染色体异常是导致无精子症及严重少精子症的重要因素之一,对无精子症及严重少精子症患者行染色体检查是十分必要的。

Objective： To investigate the relationship between chromosome abnormality disorders and azoospermia and severe oligospermia.Methods： Peripheral blood samples were obtained from 225 men with azoospermia and severe oligospermia and karyotyped on the peripheral blood lymphocytes by Giemsa banding.Results： Sixty-four abnormal chromosomal karyotypes were detected in 225 patients（28.44%）,in which sex chromosome abnormalities were seen in 39（including 2 cases of sex reversal）（39/64;60.94%）,euchromosome abnormalities in 4（4/64;6.25%） and chromosome polymorphism in 21（21/64;32.81%）.Conclusion： The findings suggest that chromosomal abnormalities are causative factors of azoospermia and severe oligospermia,therefore,genetic testing should be offered to the men with azoospermia and severe oligospermia.