PTEN、Ki-67在脑胶质瘤中的表达及意义

Expression and significance of PTEN and Ki-67 in human brain glioma

目的:对抑癌因子PTEN、Ki-67及其在人脑胶质细胞瘤中的表达进行相关研究。方法:免疫组化法检测80例已确诊为脑胶质瘤中PTEN、Ki-67的表达,结合临床随访资料进行分析。结果:PTEN阳性染色定位于细胞核,高分化脑胶质瘤(Ⅰ～Ⅱ级)的阳性表达率明显高于低分化肿瘤(Ⅲ～Ⅳ级)的阳性表达率。Ki-67阳性染色定位于细胞核,高分化脑胶质瘤(Ⅰ～Ⅱ级)阳性表达率明显低于低分化肿瘤(Ⅲ～Ⅳ级)。PTEN和Ki-67的表达与患者的年龄、性别、肿瘤的大小及发病部位均无相关性但二者之间的表达呈负相关。结论:PTEN、Ki-67在脑胶质瘤中的异常表达与肿瘤的分化程度相关,PTEN、Ki-67基因异常在脑胶质瘤的形成及其发展中有着拮抗作用。可以认为PTEN突变或缺失在人脑胶质瘤的发生发展中起重要作用,Ki-67可作为反映胶质瘤细胞增殖潜能的指标。

Objective：To explore the expression of PTEN and Ki-67 and significance in human brain glioma incidence. Methods：Immunohistochemical was used to detect the protein expression of PTEN and Ki-67 in brain glioma and the findings were analyzed on basis of follow-up information. Results：Both PTEN and Ki-67 were positively expressed in the nucleus,and the former had a higher positive expression in well-differentiated brain gliomas（Ⅰ-Ⅱ）,while the latter was positively over-expressed in those of poorly differentiated（Ⅲ-Ⅳ）（P〈 0.05）.The expression of PTEN was negatively correlated with Ki-67 and both protein expression suggested no relationship with ages,sexes,tumor size and tumor location. Conclusion：Aberrant expression of PTEN and Ki-67 in brain glioma is associated with the degree of tumor cell differentiation,and PTEN acts as the antagonist of Ki-67,suggesting that mutation or deletion of PTEN gene plays an important role in the glioma formation and development and Ki-67 can be applied as an indicator to evaluate the state of cell proliferation.