微生物转化生产2-苯乙醇的原位产物转移技术研究进展

In situ product removal used in 2-phenylethanol production by microbial conversion

阐述了微生物转化法生产天然2-苯乙醇的代谢途径以及2-苯乙醇对微生物的毒性即产物抑制作用,而原位产物转移技术(ISPR技术)可有效降低产物抑制作用,提高2-苯乙醇产量。着重介绍了微生物转化生产2-苯乙醇的ISPR技术的研究现状,比较总结了萃取、吸附与基于膜分离的渗透蒸发和渗透萃取技术的优缺点,提出今后的研究重点应是将开发ISPR技术与培育高产量菌株和高效发酵工艺相结合,利用相对简单的设备及后处理技术即可显著提高2-苯乙醇的产率,早日实现微生物转化生产2-苯乙醇的工业化。

2-phenylethanol（2-PEA）is an aromatic alcohol with rose fragrance,which has been widely used as an essence component in fine chemical and food industries.Compared with the low quality 2-PEA by chemical synthesis and the low yield and high price 2-PEA by plant extraction,2-PEA production by microbial conversion has been increasingly valued,but the product inhibition of 2-PEA to microbes is a problem.In situ product removal（ISPR）techniques can effectively improve 2-PEA yield by reducing product inhibition.The progress of ISPR techniques used in 2-PEA production by microbial conversion was introduced including the extraction,adsorption and membrane-based pervaporation and penetration extraction.The combination of ISPR techniques,high-efficient fermentation process and strain screening for high yield,the development of relatively simple instruments and post-processing techniques are suggested to be the research focus,so as to significantly improve the yield of 2-PEA and realize the industrialization of 2-PEA production by microbial conversion.