类胡萝卜素在锦鲤组织器官中的沉积与分布

Deposition and distribution of carotenoids in different tissues of ornamental carp Cyprinus carpio

在6个90 L蓝色方形水槽中各放养22尾锦鲤Cyprinus carpio幼鱼,初始体质量为(7.31±0.12)g,在水温相同的环境条件下先用添加虾青素(130 mg/kg)和螺旋藻(质量分数为12%)2组饲料饲喂21 d,再用没有添加虾青素和螺旋藻的基础饲料饲喂20 d,每个试验组设3个平行,研究了虾青素和螺旋藻对锦鲤各组织器官中类胡萝卜素含量的影响以及在锦鲤各组织器官中的代谢途径。结果表明:投喂添加着色剂的饲料后,锦鲤各组织器官中的类胡萝卜素含量均随投喂时间的延长而升高;类胡萝卜素在锦鲤各组织器官中的含量从高至低依次为皮肤>鳍>肝胰脏>眼>肌肉,皮肤和鱼鳍是锦鲤色素沉积的主要部位;锦鲤的眼、肌肉、肝胰脏中色素的沉积随着饲养时间的延长变化不显著,而锦鲤的背鳍、尾鳍和皮肤随饲养时间的延长变化较显著;停喂添加着色剂的饲料后,锦鲤各组织器官中的类胡萝卜素含量均随停喂时间的延长而降低。以虾青素作为着色剂时,锦鲤体表类胡萝卜素含量于饲喂第9天开始显著升高;而以螺旋藻为着色剂时,锦鲤体表类胡萝卜素含量于饲喂第13天开始显著升高,锦鲤对虾青素的沉积速度比螺旋藻快。

A feeding trial was conducted to investigate the effects of dietary astaxanthin and Spirulina on the carotenoids contents and the metabolic pathways in different tissues of ornmmental carp Cyprinus carpio with initial body weight of（7.31±0.12）g.The fish were stocked into 90 L blue plastic tanks at a rate of 22 individuals/tank and fed the diets containing astaxanthin（130 mg/kg） and Spirulina（12%） for 21 days,and then fed the diet without astaxanthin and Spirulina for 20 days.The results showed that the carotenoid contents in different tissues of the fish were found to increase with increase in feeding period.The total deposition of the carotenoid content was mainly concentrated in the skin and fin,following in the hepatopancreas,eyes,and muscle of the fish.There were significant differences in carotenoids contents in eyes,muscle and hepatopancreas with increasing feeding period,but in the dorsal fin,caudal fin and skin of the fish significant difference with time increase.The total carotenoid contents in various tissues were found gradual decrease in the fish fed the basal diets as the feeding time prolonged.The total carotenoid contents in body surface was found significant increase 9 days after the fish fed the diet containing astaxanthin.In the fish fed the diet containing Spirulina,however,the significant increase in total carotenoid contents in body surface was observed 13 days later.