摘要
采用共培养的方法,研究了角叉菜Chondrus ocellatus新鲜组织、干粉末、水溶性抽提液和培养水的过滤液对米氏凯伦藻Karenia mikimotoi和微小原甲藻Prorocentrum minimum的克生效应。结果表明:新鲜角叉菜、干粉末及水溶性抽提液对米氏凯伦藻和微小原甲藻的生长具有显著的抑制作用(P<0.05),且新鲜组织和干粉末在较高浓度下对赤潮微藻的生长具有致死效应;采用一次性及半连续培养方式时,角叉菜的培养水过滤液对微小原甲藻的生长具有显著的抑制效应(P<0.05),而采用一次性添加方式时,角叉菜的培养水过滤液对米氏凯伦藻的生长没有显著的抑制作用(P>0.05),但采用半连续添加方式时,米氏凯伦藻的生长受到了显著抑制(P<0.05),表明角叉菜分泌了非常容易降解的克生物质。
The allelopathic effect of fresh tissue,dry powder,culture filtrate water,and water-soluble extract of sea weed Chondrus ocellatus on the growth of algae Karenia mikimotoi and Prorocentrum minimum was studied using co-culture method.The results showed that fresh tissue,dry powder and water-soluble extract of the sea weed had significant inhibition on the growth of the algae,and the fresh tissue,and dry powder had lethal effect on the algae at a higher concentration.Under a one-time and semi-continuous culture,the sea weed had significant inhibitory effect on the growth of P.minimum.There was no significant(P〈0.05) inhibitory effect on the growth of K.mikimotoi in the cultivation liquid water filtration of the sea weed in the one-time mode supplemented once.In the semi-continuous mode supplementation,the growth of K.mikimotoi was found to be significant inhibition(P〈0.05),indicating that the sea weed produces the degradable allelochemicals.
出处
《大连海洋大学学报》
CAS
CSCD
北大核心
2012年第1期27-31,共5页
Journal of Dalian Ocean University
基金
海洋公益性行业科研专项(200805069)
辽宁省博士启动基金资助项目(20081076)
辽宁省教育厅高校科研项目(2009A174)
大连海洋大学引进人才科研启动项目(015636)
关键词
角叉菜
米氏凯伦藻
微小原甲藻
克生作用
Chondrus oceUatus
Karenia mikimotoi
Prorocentrum minimum
allelopathic effect