摘要
为了给气候变化背景下的竹林培育应对策略提供理论依据,以四季竹Oligostachyum lubricum为材料,运用开顶式气室(OTCs)模拟当前环境背景大气臭氧(O3)体积分数(NF,40~45 nL.L-1)倍增1倍(TR-1,92~106 nL.L-1)和倍增2倍(TR-2,142~160 nL.L-1)情景,分析叶片光合色素、可溶性蛋白、可溶性糖和脂质过氧化及抗氧化酶等生理指标的变化。结果表明:大气臭氧体积分数倍增90 d时,与NF比较,TR-1和TR-2处理的四季竹叶片叶绿素、类胡萝卜素、可溶性蛋白和超氧化物歧化酶(SOD)活性均显著降低,而叶片相对电导率、丙二醛(MDA)和过氧化物酶(POD)活性均显著升高,而叶片可溶性糖仅TR-2较NF显著下降;叶片相对电导率和丙二醛TR-2显著高于TR-1,而叶片可溶性糖TR-2显著低于TR-1。说明大气臭氧体积分数倍增能引起四季竹叶片细胞膜脂过氧化,膜结构遭到破坏,影响四季竹的正常生长,表现出伤害效应。
This research was conducted to provide theoretical evidence for bamboo cultivation during this time of possible climate change.Open-top chambers(OTCs) were employed to determine the effects of elevated ozone on photosynthetic pigment content,soluble protein content,soluble sugar content,lipid peroxidation,and anti-oxidation enzymes in the leaves of Oligostachyum lubricum using a split-plot design with treatments of Control(NF),92-106 nL·L-1 of O3 concentration(TR-1),and 142-160 nL·L-1 of O3 concentration(TR-2),three replications.Results showed that,after 90 d,compared with NF,in treatments TR-1 and TR-2 there were significant decreases in the contents of chlorophyll(P = 0.002 4),carotenoids(P = 0.049 4),and soluble protein(P = 0.031 5),as well as superoxide dismutase(SOD) activity(P = 0.033 0) with significant increases in relative electrical conductivity(P = 0.013 5),peroxidase(POD) activity(P = 0.039 6),and malondialdehyde(MDA) content(P = 0.000 4).Soluble sugar content decreased significantly(P = 0.003 7) only in treatment TR-2.Also,in leaves of TR-2 compared to TR-1,there was a significant increase in electrical conductivity(P = 0.044 8) and MDA content(P = 0.000 2),but a significant decrease in soluble sugar content(P = 0.001 5).Thus,elevated ozone concentrations intensified the damage to membrane lipids and destroyed the structure of cell membranes,thereby affecting normal growth of O.lubricum.
出处
《浙江农林大学学报》
CAS
CSCD
北大核心
2012年第1期12-16,共5页
Journal of Zhejiang A&F University
基金
中央级公益性科研院所基本科研业务费专项资金(RISF6915)
国家林业公益性行业科研专项(201004008)
浙江省省院合作项目(2010SY01)
关键词
植物学
四季竹
臭氧体积分数倍增
光合色素
膜脂过氧化
抗氧化酶
botany
Oligostachyum lubricum
elevated ozone concentration
photosynthetic pigment
membrane lipid peroxidation
antioxidant enzymes