金线莲离体快繁及植株再生

Rapid Propagation and Regeneration In vitro in Anoectochilus roxburghii

以金线莲野生植株茎段为外植体,在无菌系建立、增殖和生根培养时进行了适宜培养基的筛选试验,结果表明:以MS为诱导培养基建立无菌系效果较好;以MS+KT 1.0 mg/L(单位下同)+IAA 0.2 mg/L+0.15%活性炭能有效增殖,匍匐茎节部白色芽点多,易生成片状丛生芽块;在1/2 MS+IAA 2.0+0.15%活性炭的培养基上生根率达95%以上。同时开展了组培苗移栽试验,以泥炭土∶腐殖土∶蛭石=1∶1∶1配比为组培苗移栽适宜基质,在可控的设施环境下或透光度50%左右的林下移栽,成活率达87.6%。

With the stem segments of wild Anoectochilus roxburghii plants as explants,the experiments on the selection of suitable medium for induction,proliferation and rooting were carried out.The results showed that the suitable induction medium was MS;the proliferation medium was MS＋KT 1.0 mg/L ＋IAA 0.2 mg/L ＋0.15% activated carbon,creeping section part has white buds and with more generate block buds flakes;and the rooting medium was 1/2 MS＋IAA 2.0 mg/L ＋0.15% activated carbon,then the rooting rate can reach above 95%.Meanwhile,the tests of tissue cultural plantlets transplanting were developed,and the results were demonstrated that the mixed material with peat：muck：vermiculite=1∶1∶1 was suited for the transplanting in forests with a controlled facilities environment,or about 50% transmittance,and the survival rate can reach 87.6%.