不同方式获取精子对ICSI治疗后妊娠结局分析

Analysis of pregnancy outcome due to different ways to get sperms after intracytoplasmic sperm injection

目的分析和比较3种不同获取精子方式行ICSI后的妊娠结局。方法回顾性分析本中心2009年2月至12月进行ICSI治疗的207例患者，其中严重少弱精子患者108例、当天受精失败81例和经皮附睾穿刺术（PESA）18例，比较3组的受精率、卵裂率、优质胚胎率和妊娠率。结果3组的受精率、卵裂率、优质胚胎率无明显差异，其中受精失败组行补救ICSI妊娠率较高，但与其他两组比较没有统计学意义。结论严重少弱精子症、附睾穿刺和补救ICSI获得的精子，受精率和优质胚胎率，妊娠率相似，不同来源和生理功能的精子不影响ICSI的治疗效果。

Objective To analyze the pregnancy outcome after intracytoplasmic sperm injection （ICSI） with three ways to get sperm. Methods The rate of fertilization, cleavage and pregnancy in 207 patients treated with ICSI was reviewed from January to December 2009. They were divided into three groups according to sperm source, i.e. the severe oligo-asthenospermia group （108 cases）, percutaneous epididymal sperm aspiration group （18 cases）, and fertilization failure group （81 cases）. Results There was no significant difference among the fertilization, cleavage and pregnancy rates in the three groups. Conclusion The rate of fertilization, super-embryo and pregnancy is similar among the three groups The efficacy of the ICSI treatment is not affected by the spermatozoa from different sources and physiological functions.