摘要
目的建立乳安凝胶膏剂中淫羊藿苷、延胡索乙素等4种化学成分的HPLC测定方法。方法 Hed-era C18柱;以乙腈(A)-0.1%磷酸水溶液(B)为流动相进行梯度洗脱,流速:1mL.min-1,柱温:30℃,检测波长:280nm。结果 4种成分均能达到基线分离,各成分在其线性范围内呈良好的线性关系,加样回收率在95%～105%。结论上述方法可用于乳安凝胶膏剂的含量测定。
Objective To develop a HPLC method for simultaneous determination of Icariin,corydalis B,eugenol and cinnam aldehyde in Ru-an Cataplasm.Methods The chromatographic separation was achieved on a Hedera C18 column with a mobile phase which was composed of acetonitrile（A） and 0.1% phosphoric acid（B） for gradient elution.The flow rate was 1.0mL·min-1.The column temperature was 30℃,and the detection wavelength was set at 280nm.Results The results showed that four effective components were separated well and showed good linearity.The average recoveries were between 95%-105%.Conclusion The method can be used for the quality control of Ru-an Cataplasm.
出处
《中国药事》
CAS
2012年第1期25-27,共3页
Chinese Pharmaceutical Affairs
基金
江苏省中医药局科技项目周期性中药巴布剂外敷治疗乳腺增生病的临床研究(编号LZ09044)
