孔雀草组培繁殖技术研究

Tissue Culture and Propagation Technology of Tagetes patula L.

[目的]对孔雀草组培繁殖技术进行研究。[方法]利用组织培养技术,在MS培养基中加入不同质量分数的6-苄基氨基腺嘌呤(6-BA)和萘乙酸(NAA),比较各培养基对孔雀草快速繁殖的影响。[结果]以茎尖或茎段为外植体进行组织培养,适宜的消毒时间为8min;茎尖分化效果好于茎段;生长调解剂含量高时,出愈率高;以MS+NAA 0.1 mg/L+6-BA 1.5 mg/L培养基为继代增殖培养基,继代周期为4周;试管苗在1/2MS+NAA 0.2 mg/L培养基上生根率最高(97%),且根系较发达。[结论]为孔雀草的工厂化育苗提供了技术支撑。

[Objective] This study aimed to investigate the tissue culture and propagation technology of T.patula L..[Method] By using tissue culture technology,different mass fractions of 6-BA and NAA were added to MS medium,to compare the effect of different culture medium on the rapid propagation of T.patula L..[Result] Shoot tips or stem segments of T.patula L.were used as explants for tissue culture with an appropriate sterilization time of 8 min;differentiation effect of shoot tips was better than that of stem segments;callus generation rate was high with the high content of growth regulators;MS medium containing 0.1 mg/L of NAA and 1.5 mg/L of 6-BA was used for subculture proliferation with a subculture period of 4 weeks;rooting rate of plantlet was the maximum（97%） in 1/2MS medium containing 0.2 mg/L of NAA,and the root system was relatively developed.[Conclusion] This study provided technical support for the industrialized seedling breeding of T.patula L..