摘要
[目的]优化根瘤农杆菌介导的水稻转基因方法。[方法]通过对经典水稻转基因方法的优化和改进,高效获得转基因水稻苗。[结果]使用成熟胚,采取升汞灭菌,侵染时农杆菌的OD600值为0.1时,转化效率最高,较原方法提高约23%。[结论]该研究为以根瘤农杆菌介导的水稻转基因研究提供了技术参考。
[Objective] This study aimed to optimize the method for Agrobacterium-mediated transformation in rice.[Method] Transgenic rice seedlings were obtained by optimizing and modifying the classical transformation methods.[Result] Mature embryos were adopted and sterilized by HgCl2.Transformation efficiency had achieved the maximum when OD600 was 0.10,which had increased by 23%,comparing with previous method.[Conclusion] The study provided a technical reference for relative researches of Agrobacterium-mediated transformation in rice.
出处
《安徽农业科学》
CAS
2012年第7期3867-3867,3880,共2页
Journal of Anhui Agricultural Sciences
基金
农业部转基因新品种培育重大专项(2009ZX08009-071B)
关键词
水稻
转基因
组织培养
Rice
Transgene
Tissue culture