番茄组织培养与试管苗繁育

Inducing of Adventitious Buds from Tomato Callus and Rooting

[目的]建立番茄的高效再生体系,为下一步进行番茄叶绿体遗传转化研究奠定基础。[方法]通过培养经无菌处理的番茄种子获得无菌苗,切取无菌苗的叶片置于附加有激素组合为3.0 mg/L 6-BA+0.3 mg/L IAA的MS培养基中诱导愈伤组织产生,再利用愈伤组织,比较不同浓度激素组合对番茄愈伤组织分化不定芽与不定芽生根的影响。[结果]MS+2.0 mg/L 6-BA+0.3 mg/L IAA+30 mg/L蔗糖培养基对诱导愈伤组织分化不定芽的效果最佳;1/2MS+1.0 mg/L IAA培养基对生根最好。[结论]适宜激素浓度的选择是番茄高效再生体系建立的关键。

[Objective] The aim was to establish efficient regeneration system of tomato so as to prepare for transformation of tomato genetic.[Method] Cultivate tomato seeds processed aseptically to get plantlets.Then,cultivate leaves cut from plantlets in the MS with 3.0 mg/L 6-BA＋0.3 mg/L IAA to induce callus.Finally,compare different hormones and concentration on induction of adventitious buds from tomato callus and rooting.[Result] The best medium was MS ＋ 2.0 mg/L 6-BA＋0.3 mg/L IAA＋30 mg/L sugar,in which many embryo calli were produced;The best medium for rooting was 1/2MS ＋ 1.0 mg/L IAA.[Conclusion] Appropriate choice of hormone concentrations is the key to establish efficient regeneration system for tomato.