木薯种质资源遗传多态性ISSR分子标记的研究

Research on Genetic Polymorphism of Cassava Germplasms and ISSR Molecular Markers

[目的]研究广西壮族自治区亚热带作物研究所保存的39份木薯种质资源遗传多样性。[方法]筛选出对木薯具有较好多态性和效果的ISSR引物,应用筛选出的引物对39份木薯种质资源进行PCR扩增,并对扩增条带进行统计和分析。同时根据品系间的遗传相似系数,利用UPGMA法进行聚类分析。[结果]筛选出10个ISSR引物,每个引物检测等位基因3～9个,平均为7个,获得70个扩增带形,扩增产物的片段大小范围在150～2 000 bp之间;聚类分析得到,在遗传相似系数0.67上将39个木薯品系分为2个类群,同时聚类分析结果表明,木薯之间的遗传距离基础非常狭窄,遗传相似系数大多在0.80～1.00之间。[结论]为我国优质木薯的育种奠定基础。

[Objective] This study aimed to analyze the genetic polymorphism of 39 cassava varieties（lines） saved in Guangxi Zhuang Autonomous Region Research Institute for Subtropical Crops.[Method] ISSR primers with good polymorphisms and amplification effects in cassava were selected for PCR amplification of 39 cassava germplasms,statistics and analysis of the amplified bands were conducted.In addition,cluster analysis was conducted by using UPGMA method based on the genetic similarity among cassava varieties（lines）.[Result] 10 of ISSR primers were selected.A total of 70 clear electrophoretic bands were amplified,each primer had amplified 3-9 electrophoretic bands,with an average of 7,and the length of amplified bands ranged from 150 bp to 2 000 bp;cluster analysis showed that 39 cassava varieties（lines） were clustered into two categories by the similarity coefficient of 0.67;in addition,the genetic distance among cassava was very narrow,with genetic similarity coefficients ranged between 0.80 and 1.00.[Conclusion] This study laid a foundation for breeding of high-quality cassava in China.