基于ISSR标记的地被竹种的遗传多样性研究

Study on Genetic Diversity of Turf Bamboo Based on ISSR Marker

[目的]利用ISSR分子标记技术对地被竹种的遗传多样性进行分析,旨在为地被观赏竹种的育种和栽培推广提供理论和技术基础。[方法]以从法国引进的优良地被竹种和国内部分观赏地被竹种为材料进行ISSR分析,并以获得的ISSR分子标记为依据对10种地被竹材料进行聚类分析。[结果]21条ISSR引物扩增得到条带清晰、重复性好、多态性高的条带201条,多态性比率93.1%;不同地被竹种之间的相似系数在0.275～0.571之间,平均相似系数为0.357;根据ISSR标记的结果,采用UPGMA聚类分析方法可将10个不同地被观赏竹种分为3个类群。[结论]该研究表明不同来源的地被竹有较高的遗传多样性,为地被竹种的育种和栽培推广提供了理论依据。

[Objective] This study aimed to analyze the genetic diversity of turf bamboo species by using ISSR molecular marker technology.[Method] Excellent turf bamboo species imported from France and domestic ornamental turf bamboo species were used as experimental materials for ISSR analysis,cluster analysis was conducted on 10 species of turf bamboo materials based on the obtained ISSR molecular markers.[Result] A total of 201 clear bands with good repeatability and high polymorphism were amplified with 21 ISSR primers,with a polymorphism rate of 93.1%;similarity coefficients between different turf bamboo species ranged from 0.275 to 0.571,with an average similarity coefficient of 0.357;according to the results of ISSR markers,10 different ornamental turf bamboo species were divided into three categories by using UPGMA cluster analysis method.[Conclusion] Turf bamboo with different sources had relatively high genetic diversity,this study had provided theoretical and technical basis for the breeding,cultivation and promotion of ornamental turf bamboo.