博落回根提取物对黄粉虫的触杀活性

Contact Toxicity of Root Extracts from Macleaya cordata against Tenhrio motlitorlin(L.)

[目的]筛选博落回根的活性部位。[方法]采用浸渍法,研究了博落回根乙醇粗提取物及其石油醚、乙酸乙酯、正丁醇和水4种萃取物对2、6龄黄粉虫的触杀活性。[结果]随着提取物浓度的升高,黄粉虫的校正死亡率升高,当浓度为100 mg/ml时,乙酸乙酯萃取物对2龄黄粉虫的触杀活性最高,48 h校正死亡率达80.95%,LC50为65.532 9 mg/ml,杀虫活性整体表现为:乙酸乙酯层>石油醚层=正丁醇层>总提取物(乙醇)>水层;乙醇提取物和石油醚萃取物对6龄黄粉虫的触杀活性最高,48 h校正死亡率分别达71.42%和66.67%,LC50分别为83.899 8和86.687 7 mg/ml,杀虫活性整体表现为:总提取物(乙醇)>石油醚层>乙酸乙酯层>正丁醇层>水层。[结论]博落回根杀虫活性成分主要分布在石油醚和乙酸乙酯所在极性范围内,可对该部位进一步分离、鉴定。

[Objective] The study was to screen the active part from roots of Macleaya cordata.[Method] Dipping method was used to study the contact toxicity of ethanol extract and 4 kinds of extracts by petroleum,ethyl acetate,n-butyl alcohol and water from the root of M.cordata on 2nd and 6th instars larvae of T.motlitorlin（L.）.[Result] The corrected mortality of T.motlitorlin increased with the increase of extract concentration.When the concentration was 100 mg/ml,the contact toxicity of ethyl acetate extract against 2nd instar larvae of T.motlitorlin was the highest,the corrected mortality after 48 h reached 80.95%,and LC50 was 65.532 9 mg/ml.The overall performance of insecticidal activity was as follows： ethyl acetate layer petroleum layer = n-butyl alcohol layer total extracts（ethanol） water layer.The contact toxicity of ethanol extract and petroleum extract against 6th instar larvae of T.motlitorlin was the highest,and the corrected mortality after 48 h reached 71.42% and 66.67%,LC50 were 83.899 8 and 86.687 7 mg/ml,respectively.The overall performance of insecticidal activity was as follows： total extracts（ethanol） petroleum layer ethyl acetate layer n-butyl alcohol layer water layer.[Conclusion] The insecticidal active components of M.cordata root were distributed within the polarity range of petroleum and ethyl acetate,and the part could be further separated and identified.