摘要
[目的]对飞扬草地上部分进行化学成分研究。[方法]利用正反相硅胶和Sephadex LH-20对飞扬草的化学成分进行分离、纯化,并利用MS,NMR等光谱技术鉴定化合物结构,采用流式细胞术对化合物1,4,5,6进行了小鼠脾细胞免疫刺激活性测试。[结果]从飞扬草地上部分分离得到6个化合物,经鉴定分别为邻苯二甲酸二异丁基酯(化合物1)、邻苯二甲酸二乙基己基酯(化合物2)、高车前素(化合物3)、过氧化乙酰(化合物4)、槲皮素(化合物5)和没食子酸(化合物6),所有测试化合物均未发现有明显的免疫刺激活性。[结论]成分1~3为首次从飞扬草中分离得到,4为首次从天然产物中得到。该研究为从飞扬草中寻找生物活性物质提供了参考依据。
[Objective] The aim was to study the chemical constituents in Euphorbia hirta.[Method] The constituents were isolated and purified by various chromatography methods including normal phase and reverse phase silica gel as well as Sephadex LH-20 gel column,and then elucidated by spectroscopic analysis such as MS and NMR etc.Compounds 1,4,5 and 6 were evaluated for their immunostimulatory activity against splenic cell of mouse by FCM.[Result] Six compounds were isolated from Euphorbia hirta,and identified as diisobutyl-O-phthalate(compond 1),diethylhexyl phthalate(compond 2),hispidulin(compond 3),acetyl peroxide(compond 4),quercetein(compond 5) and gallic acid(compond 6).All compounds were inactive against splenic cell of mouse.[Conclusion] Compounds 1,2 and 3 were isolated from this plant for the first time,and compound 4 was the first naturally occurring compound.This study provided reference for isolating bioactive substances from Euphorbia hirta.
出处
《安徽农业科学》
CAS
2012年第7期4060-4062,共3页
Journal of Anhui Agricultural Sciences
基金
国家自然科学基金(21162044)
云南省自然科学基金(2009CD051)
云南省中青年学术后备人才项目(2010-CI040)
关键词
大戟科
飞扬草
化学成分
免疫活性
Euphorbiaceae
Euphorbia hirta
Constituents
Immunological activities