稻瘟病菌NAD(H)激酶Mopos5互作蛋白的筛选

Screening of Interaction Proteins of NAD(H) Kinase Mopos5 in Magnaporthe oryzae

为了探索Mopos5可能参与的信号网络,了解其作用的分子机制,利用酵母双杂交系统筛选了稻瘟病菌cDNA文库,获得了6个与Mopos5互作的蛋白;结合生物信息学预测分析和相关文献报道,发现这些候选互作蛋白很可能参与调控了细胞骨架建成、细胞运动、细胞分裂、细胞老化与死亡、线粒体功能维持、胁迫防御反应、物质与能量代谢和信号传导等多种多样的生物学进程。进一步分析这些互作蛋白的生物学功能及与Mopos5的互作机制,对于了解Mopos5可能参与的信号网络及其调控稻瘟病菌在寄主组织内定殖和扩展的分子机制具有重要的意义。

In order to explore the signal networks in which Mopos5 might involve and study the molecular mechanism,cDNA library was screened by using yeast two-hybrid system,and six interaction proteins were obtained.According to bioinformatics prediction and relevant research papers,these candidate interaction proteins were likely to participate in various biological processes,including cytoskeleton morphogenesis,cell movement,cell division,cell ageing and death,maintenance of mitochondrial function,defense response to environmental stress,material and energy metabolism and signal transduction.Further analysis of the biological functions of these interaction proteins and their interaction mechanisms with Mopos5 would be of great significances to make out the signal networks in which Mopos5 might involve and even its molecular regulation mechanisms of colonization and expansion of M.oryzae in rice tissues.