昆虫取食诱导棉花和玉米多酚氧化酶和过氧化物酶的活性研究

The Study on the Activities of Polyphenol Oxidase and Peroxidase in Cotton and Corn Induced by Insects

为了明确昆虫取食诱导植物间的通讯作用机制,研究了棉蚜取食和机械损伤诱导棉花及其邻近玉米,以及玉米螟取食和机械损伤诱导玉米及其邻近棉花的诱导防御机制。利用分光光度计法测定不同处理棉花和玉米的多酚氧化酶及过氧化物酶的活性。结果表明,棉蚜取食和机械损伤诱导棉花多酚氧化酶和过氧化物酶的活性增加,棉蚜取食诱导棉花多酚氧化酶的活性是对照的1.61倍,机械损伤诱导棉花过氧化物酶的活性是对照的1.84倍,2种处理与对照相比差异均显著。玉米螟取食和机械损伤诱导玉米多酚氧化酶的活性增加但与对照比差异都不显著,而其相应过氧化物酶的活性显著高于对照,分别为对照的4.79倍和3.56倍。棉蚜取食和机械损伤诱导相应邻近健康玉米及玉米螟取食和机械损伤诱导其相应健康棉花的多酚氧化酶和过氧化物酶活性都没有增加。由此认为,多酚氧化酶和过氧化物酶在昆虫取食诱导的棉花和玉米通讯中的作用不明显。

In order to clarify plant communication induced by herbivory,cotton and corn were used as materials to study plant communication in different treatments,including cotton induced by aphid and artificial wound and its corresponding corn nearby,and corn induced by corn borer feeding and artificial wound and its corresponding cotton nearby.The activities of polyphenol oxidase and peroxidase were detected using spectrophotometry in different treatments.The results showed as followings：the activities of polyphenol oxidase and peroxidase increased in cotton induced by aphid infestation and artificial wound.The activity of polyphenol oxidase in cotton infested by aphid was 1.61 fold the control and the deference was significantly.The activity of peroxidase in wounded cotton was significantly increased and 1.84 fold the control.The activities of polyphenol oxidase increased in corn induced by mechanical wounding and corn borer feeding compared to the control,but the difference was not significantly,while the activities of peroxidase in corn induced by mechanical wound and corn borer feeding increased significantly,and the induced fold was 4.79 and 3.56 respectively.The activities of polyphenol oxidase and peroxidase in the corresponding receiver seedlings were not induced.Polyphenol oxidase and peroxidase didn’t play an important role in cotton-corn communication induced by herbivory.