HPV16E7基因沉默对宫颈癌细胞系CaSki细胞增殖的影响

The Effect of HPV16E7 Gene Silencing on the Proliferation of Cervical Cancer Cell Line CaSki

[目的]探讨脂质体转染HPV16E7 siRNA对人宫颈癌CaSki细胞增殖的影响。[方法]人工合成抑制HPV16E7基因的siRNA片段,通过脂质体转染到CaSki细胞内,显微镜下观察其形态学变化;流式细胞术检测各组细胞周期变化;RT-PCR检测HPV16E7 mRNA的表达;Western blot检测HPV16E7蛋白表达。[结果]转染siRNA后的CaSki细胞,细胞增殖受到显著抑制,HPV16E7 mRNA表达显著下降,HPV16E7蛋白水平显著下降。[结论]应用RNA干扰靶向抑制HPV16E7基因可以显著抑制CaSki细胞增殖。

[Purpose] To investigate the effect of liposomal transfection of HPV16E7 siRNA on the proliferation of cervical carcinoma cell line CaSki. [Methods] HPV16E7 siRNA was synthesized and transfeeted into CaSki cell by liposome. Cell morphological change was observed with mi- croscopy, and cell proliferation index was detected by flowcytometry. HPV16E7 mRNA expression was determined by RT-PCR and protein level of HPV16E7 was determined by Western blot. IRe- suits] For the CaSki cell transfected with siRNA, cell proliferation was inhibited significantly, the expression of HPV16E7 mRNA and protein level of HPV16E7 was significantly decreased. [ Conclusion ] HPV16E7 siRNA can inhibit the proliferation of cervical carcinoma cell line CaSki.