摘要
目的建立视舒茶的质量标准。方法采用薄层色谱法对处方中玄参、红花、甘草进行定性鉴别,采用高效液相色谱法对芍药苷含量进行测定。结果薄层色谱法鉴别出玄参、红花、甘草,且专属性强、重复性好;高效液相色谱法测定芍药苷含量,进样量在0.04108~0.12324/μg/mL之间呈良好的线性关系,平均回收率为99.29%,RSD为0.64%。结论该方法准确、可靠重现性好。
Objective To establish the quality standard for Shishu Tea. Methods Using TLC to identify Scrophulariae Radix, Carthami Flos and Glycyrrhizae Radix et Rhizoma. Content of paeoniflorin was determined by HPLC. Results Scrophulariae Radix, Carthami Flos and Glycyrrhizae Radix et Rhizoma were identified by TLC. The method was specific and reproducible. Paeoniflorin showed a good linear in the range of 0.041 08 -0.123 24 μg/mL ,and the average recovery was 99.29%with RSD=O.64%.Conclusion The method was accurate, reliable and reproducible, which could be applied to the quality control of Shishu Tea.
出处
《现代中药研究与实践》
CAS
2012年第1期64-66,共3页
Research and Practice on Chinese Medicines