摘要
目的:研究细胞因子TNF-α和针对DNA甲基化转移酶的siRNA对Burkitt淋巴瘤细胞株Raji细胞的癌-睾丸抗原表达情况的影响。方法:1.加入不同终浓度的细胞因子TNF-α,与Raji细胞株共培养,在不同的时间点检测癌-睾丸抗原表达情况的改变。2.转入针对DNA甲基化转移酶的siRNA,检测癌-睾丸抗原表达情况的改变。结果:在TNF-α作用后,一些细胞组会出现SSX-1表达上调,而siRNA干扰作用后,转染针对DNMT1和DNMT3b的细胞组或受5-aza-dC作用的细胞组的PRAME发生再表达。结论:TNF-α和siRNA都能诱导某些癌睾丸抗原的表达或使其表达增强。
Objective： To investigate the effect of TNF-α and RNA interference of DNMTs on the expression file of Can-cer-Testis Antigens in Burkitt lymphoma cell line Raji.Methods： The expression file of Cancer-Testis antigens mRNA in Raji cell treated with TNF-α and transfected with siRNA was detected by RT-PCR respectively.Results： After treated by TNF-α,the expression of SSX-1 and NY-ESO-1 were upegulated in some groups,while PRAME was re-expressed in groups transfected with siRNA towards DNMT1 and DNMT3b or 5-aza-dC.Conclusion： TNF-α and siRNA have effect on the expression level of Cancer-Testis Antigens in Raji cell line.
出处
《现代生物医学进展》
CAS
2012年第1期1-4,共4页
Progress in Modern Biomedicine
基金
国家自然科学基金资助项目(30771995)
