HSP70高表达保护低氧诱导的大鼠海马DG区神经细胞凋亡

HSP70 Over Expression Protected Neurocyte in the Area of Dentate Gyrus(DG)of Rat's Hippocampus From Hypoxia-Induced Apoptosis

目的:研究诱导HSP70高表达对低氧引起的大鼠海马DG区神经细胞凋亡的保护作用。方法:大鼠海马DG区神经细胞分别在41℃温浴2h和加入砷酸钠诱导HSP70高表达。对照低氧而不预热,并用HSP70反义寡核苷酸链抑制HSP70合成,观察HSP70与低氧大鼠海马DG区神经细胞凋亡的关系。DNA碎片法检测细胞凋亡,Western Blotting检测HSP70。结果:预热和砷酸钠都可以诱导细胞HSP70高表达;HSP70高表达可以明显减少低氧诱导的细胞凋亡。在预热前导入HSP70反义核酸,可以降低HSP70抑制细胞凋亡的作用。结论:HSP70高表达可以保护细胞由于低氧引起的细胞凋亡。

Objective： To investigate the effect of the over expression of HSP70 on neurocyte in the area of dentate gyrus（DG）of rat＇s hippocampus in hypoxia-induced apoptosis.Methods： Cells were either heated in 41℃ water for 2 hours or affected with 10μmol/L sodium arsenite for 18h to induced the over expression of HSP70.While the control cells are added HSP70 antisense oligomer which re-duced the HSP70 expression or not induced before the cells heated.Then the cells were incubated in hypoxia.Fragmentation of DNA was quantities,and HSP70 was analyzed at various times by the Western blot.Results： Both heat and sodium arsenite can induce the over expression of HSP70,which can significantly decreased hypoxia-induced apoptosis.Introduction of the HSP70 antisense oligomer prior to heating reserved the protective effect of HSP70.Conclusions： The over expression of HSP70 can protect the pre-blood cells from radi-ation-induced apoptosis.