骨髓间充质干细胞(BMSCs)移植于大鼠肝硬化模型的动物实验研究

The Experimental Study of BMSCs Transplanted Into Rat Models of Liver Cirrhosis

目的:探讨骨髓间充质干细胞应用于肝硬化模型的的实验疗效,为临床试验提供数据。方法:制作大鼠肝硬化模型,将肝硬化大鼠随机分为两组,实验组和对照组,每组25只。将骨髓间充质干细胞通过门静脉注射入实验组大鼠肝脏,术后两组大鼠仍然以0.5mL/100g的维持剂量腹腔注射40%CCl4植物油溶液,3周后处死所有实验动物,取其静脉血检测ALT、TBIL、ALB、AFP,并取大鼠肝脏做组织切片观察。结果:实验大鼠肝脏可观察到诱导分化的肝细胞。实验组大鼠死亡3只,对照组死亡2只。对照组与实验组肝功能指标ALT、ALB、TBIL、AFP有显著差异,P<0.05,具有统计学意义,AST差异无统计学意义,P>0.05。结论:BMSCs注入肝硬化大鼠模型中能有效改善肝脏的生理功能,对临床试验有很好的指导作用。

Objective： To evaluate the clinical effects of bone marrow mesenchymal stem cells used in liver cirrhosis rats and collect data for the clinical trials.Methods： Liver cirrhosis rat models were prepared and then divided randomly into two groups,experimental group and control group,25 in each group.The BMSCs were transplanted into rats＇ liver of experimental group.After surgery,all the rats were still intraperitoneally injected with 40% CCl4 solution in a dose of 0.5mL/100g.After three weeks,animals were sacrificed.Venous blood was collected to test the indexes such as ALT,TBIL,ALB and AFP,and rats＇ liver were for pathological observation.Results： Dif-ferentiated liver cells could be observed in experimental group.Three rats died in experimental group and two in control group.There were significant differences in ALT,ALB,TBIL and AFP between two groups,P0.05.However,the AST of two groups hasn＇t signifi-cant difference.Conclusion： BMSCs injected into rat models of liver cirrhosis can greatly improve liver function and also give a guide to clinical trials.