STR分型应用于病理标本污染的个体识别

Application of Individual Identification through PCR-STR for Identifying Contaminated Pathological Samples

目的:通过STR分型,对两例卵巢囊腺癌病理标本的组织细胞进行个体识别,以鉴别其中一例病理标本癌细胞是否源于污染。方法:对甲醛固定石蜡包埋组织,分别切片后在倒置显微镜下,用显微操作系统分离出不同形态的细胞团,对提取的DNA样本进行常规STR扩增检验。结果:经过STR分型比对,证实病理标本存在污染,其中一例的癌细胞来源于另一个体。结论:STR分型是用于病理石蜡包埋组织标本污染个体识别的有效手段。

Objective： To use the STR typing to identify the two pathological samples of ovary cystadenocarcinoma,in order to identify whether one of two pathological samples was contaminated.Methods： Paraffin-embedded tissues were sliced and the different morphological cells were isolated by micro-operating system on inverted microscope.Extract DNA of the isolated cells,and get the geno-type through regular PCR-STR method.Results： The genotype show the existence of contamination of the pathological samples and prove that cancer cells of sample were originated from the other sample.Conclusion： Individual identification through the PCR-STR method was the effective method to identify the contaminated pathological slice.