ICR小鼠感染日本血吸虫后Tc1和Tc2细胞的动态观察及其功能分析

Studies on dynamic changes of proportions of Tc1 and Tc2 cells during Schistosoma japonicum infection

目的观察日本血吸虫感染ICR小鼠后不同时期T淋巴细胞中杀伤性T细胞(Tc)亚型Tc1和Tc2比例的动态变化,并分析两者分别与Th1和Th2细胞比例变化的相关性。方法分别取日本血吸虫感染0、3、5、8、13周的小鼠脾脏淋巴细胞,采用流式细胞术检测各个时期Tc1、Tc2和Th1、Th2细胞亚群分别占T淋巴细胞的比例。结果与0周对照比较,感染3、5、8、13周小鼠T淋巴细胞中Tc1细胞比例显著增高(P均<0.01),感染5、8、13周小鼠T淋巴细胞中Tc2细胞比例显著增高(P均<0.01)。比较Tc1和Tc2细胞比例在相同时间点的增速发现,感染3周时Tc1细胞比例增高最显著,而感染5周时Tc2细胞比例的增高最显著。感染各个时间点CD3+T细胞中的Th1细胞比例与Tc1细胞比例平行增长,且两者呈正相关(r=0.978,P=0.004);CD3+T细胞中的Th2细胞比例与Tc2细胞比例平行增长,且两者呈正相关(r=0.974,P=0.005)。体外可溶性成虫抗原(SWA)刺激脾细胞可优势增加T淋巴细胞中的Tc1细胞比例(P<0.01),而可溶性虫卵抗原(SEA)刺激脾细胞可优势增加T淋巴细胞中的Tc2细胞比例(P<0.01)。结论日本血吸虫感染不同阶段,小鼠T淋巴细胞中的Tc1和Tc2细胞比例均显著升高,并分别与Th1和Th2细胞比例呈正相关。在感染3周时主要以Tc1细胞比例升高为主,而感染5周时主要以Tc2细胞比例升高为主。SWA可优势诱导Tc1细胞,而SEA可优势诱导Tc2细胞。

Objective To observe the dynamic changes of the proportions of T cytotoxic type 1 （Tc1） cells and T cytotoxic type 2 （Tc2） cells and analyze the correlation of them with the dynamic changes of the proportions of Th1 and Th2 cells in T lymphocytes respectively at different stages after Schistosoma japonicum infection. Method Splenocytes were prepared from spleens of mice with S. japonicum infection at different stages （0, 3, 5, 8, 13 weeks）, and the proportions of Tc1, Tc2 and Th1, Th2 cells in T cells were determined by FACS, respectively. Results Compared to the control group （0 week）, the proportions of Tc1 cells significantly increased 3, 5, 8, 13 weeks post-infection （all P values 0.01） and the proportions of Tc2 cells at different stages （5, 8, 13 weeks） post-infection also increased significantly （all P values 0.01）. During the first 3 weeks post-infection, the proportions of Tc1 cells increased most quickly than Tc2 cells did and Tc1 cells did at the other stages post-infection. However, the proportion of Tc2 cells increased most quickly 5 weeks post-infection than both Tc1 cells did and Tc2 cells did at the other stages post-infection. A positive correlation was found between the increases of the proportions of Th1 cells and Tc1 cells （ r = 0.978, P =0.004）, and a positive correlation was also found between the increases of the proportions of Th2 cells and Tc2 cells （ r = 0.974, P = 0.005）. The SWA stimulation of splenocytes of mice in vitro significantly increased the proportion of Tc1 cells （ P 0.01） while the SEA stimulation of splenocytes of mice in vitro significantly increased the proportion of Tc2 cells （ P 0.01）. Conclusions The proportions of Tc1 cells and Tc2 cells in T cells are significantly increased at different stages after S. japonicum infection. Tc1 cells are increased most quickly 3 weeks post-infection, and Tc2 cells are increased most quickly 5 weeks post-infection. A positive correlation is found between the proportions of Th cells and Tc cells in CD3＋ T cells. SWA preferentially induces the increase of Tc1 cells in CD3＋ T cells while SEA preferentially induces the increase of Tc2 cells in CD3＋ T cells in vitro.