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自噬抑制剂3-MA对乳胞素抑制胃癌BGC-823细胞增殖的影响及其机制 被引量：3

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摘要 目的探讨联合应用自噬抑制剂3-Methyladenine(3-MA)和蛋白酶体抑制剂乳胞素(Lactacystin,LAC)对胃癌细胞系BGC-823增殖的影响及其机制。方法体外培养BGC-823细胞,MTT法检测BGC-823细胞增殖率,Western印迹检测线粒体凋亡相关蛋白Cytochrome C以及内质网凋亡相关蛋白caspase-4表达水平。结果与对照组相比,蛋白酶体抑制剂LAC可以引起明显的BGC细胞增殖率的下降,联合应用3-MA可以增加其引起的细胞增殖率的下降,Western印迹结果显示LAC可以上调线粒体凋亡相关蛋白Cytochrome C以及内质网凋亡相关蛋白caspase-4表达水平,联合应用3-MA和LAC可以进一步引起表达水平的升高。结论自噬抑制剂3-MA可以增加LAC引起的细胞凋亡,通过线粒体和内质网凋亡信号通路。
作者 金镇勋 兰汝春 王菲 董长松 徐冶
机构地区 吉林医药学院附属医院消化科 吉林医药学院医学科研实验室
出处 《中国老年学杂志》 CAS CSCD 北大核心 2012年第5期977-978,共2页 Chinese Journal of Gerontology
基金 吉林省科技厅自然科学基金资助项目(No.201015240) 吉林医药学院科研基金资助项目(No.2009-10)
关键词 胃癌 3-甲基腺嘌呤 乳胞素
分类号 R737.11 [医药卫生—肿瘤]
  • 相关文献

参考文献9

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共引文献43

同被引文献25

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引证文献3

二级引证文献15

中国老年学杂志
2012年 第5期

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