成都地区婴幼儿病毒性腹泻分子流行病学研究

Molecular epidemiology of viral diarrhea in Chengdu infants and young children

目的通过分子流行病学研究成都地区婴幼儿病毒性腹泻的病原学特点，掌握本地区病原分布特征，为疫苗研制和疫情控制提供科学依据。方法采用酶联免疫吸附试验（ELISA）及逆转录聚合酶链反应（RT—PCR）对成都地区2006—2008年度376例婴幼儿腹泻粪便标本进行轮状病毒（RV）、杯状病毒（HuCV）、星状病毒（AstV）及肠道腺病毒（Adv）检测。结果RV的检出率为37．76％（142／376），其中42例进行G分型，45例进行P分型，G分型以G3型为主21例（50％），其次为G2和G1型，P分型以P[8]型为主21例，其次是P[4]型19例。RV感染主要为6～23月的婴幼儿，发病高峰在10—12月份（75．8％）。RT—PCR法检出HuCV、AstV及Adv的检出率分别为15．85％、1．64％及2．04％。结论RV是成都地区婴幼儿病毒性腹泻的主要病原，其流行的主要血清型为G3型、P[8]、P[4]型。除RV外，HuCV也是重要的病原。

Objective To investigated the molecular epidemiologic features of viral diarrhea in Chengdu infants and young children, and to establish baseline patterns of etiology, provides the scientific basis for the vaccine development and the epidemic situation control. Methods From March, 2006 to December,2008,a total of 376 infants and young children from Chengdu area hospitalized for diarrhea in Chengdu Children＇s Hospital were enrolled in this study. The stool specimen collected from each patient was tested for rotavirus （RV） , Calicivirus （CV） , astrovirus （ AstV ） and adenovirus （Adv） by using enzyme linked immunosorbent assay （ELISA） and reverse transcription-polymerase chain reaction （RT-PCR） examination. Results Among those 376 cases, there were 142 cases （ 37.76% ） of RV infections, which scattered predominantly in October to December. Among 234 cases RV negativity,there were 29 cases HuCV infections （ 15.85% ）, 5 cases AstV infections （ 1.64% ）, and 8 cases Adv infections （2. 04% ）. Conclusion RV appeared to be the main etiological agent of viral diarrhea in Chengdu infants and young children, the predominant serotype of RV were G3, P[ 8 ] and P [ 41, HuCV might be the important etiological agent besides RV.