安阳地区不同食管鳞癌标本HPV感染率的比较研究

Comparative study on the HPV infection rate of different esophageal squamous cell carcinoma in Anyang China

目的对安阳地区的食管鳞癌新鲜手术标本和石蜡标本进行HPV检测，比较两种不同标本来源食管鳞癌HPV感染检测结果，统计学分析二者之间的差异，并同其他区域检测结果进行比较，分析不同标本对检测结果的影响。方法对安阳的23例新鲜手术标本及23例石蜡标本分别进行核酸提取；分别用通用引物PCR鉴定，并用人乳头瘤病毒核酸扩增分型检测试剂盒对样品进行分型检测；比较两种标本来源检测结果并进行统计分析；比较分析检测结果与其他地区检测结果的差异。结果经检测新鲜标本HPV感染率为82．6％，其中HPV16型感染率34．8％，HPV18型感染率34．8％。石蜡包埋组织标本HPV感染率78．2％，其中HPV16型感染率30．4％，HPV18型感染率17．4％。结论首次证明两种来源标本对HPV检测结果无显著性影响，均可作为检测HPV的样本，为HPV科研检测提供更广的标本来源。

Objective Comparative and statistical analysis the HPV infection rate between fresh tissue and Paraffin-embedded Specimens of esophageal squamous cell carcinoma,and comparative the testing results with others regions. Methods Extracted the total DNA from the novel fresh tissue and Paraffinembedded Specimens; Detected the DNA by PCR with universal primer and Detected the HPV type with human papilloma virus nucleic acid amplification-based typing detection reagent kit （Hybrihio） ; Compared the statistical result from the different specimens; analyzed the result between different region. Results HPV infection rate of fresh tissue is 82.6% with HPV16 （34. 8% ） and HPV18 （ 34.8% ） , and paraffinembedded specimens is 78.2% with HPV16 （30.4%） and HPV18 （ 17.4% ）. Conclusion The results provides the first evidence that there wasn＇ t noticeable difference between HPV infection rate of the two specimens. So broader specimen source could be used for HPV testing.