黏液瘤病毒体外对胶质瘤细胞杀灭作用的研究

Study in the killing effect of Myxoma virus to C6 glioma cell in vitro

目的了解C6细胞在体外对黏液瘤病毒的易感性，并测定黏液瘤病毒对体外C6胶质瘤细胞的杀灭作用。方法以黏液瘤病毒感染体外培养的C6胶质瘤细胞，以灭活病毒作为阴性对照，5-Fu作为阳性对照，DEMD液做空白对照，应用Western—Blot法、倒置显微镜及MTT法观察各组细胞的形态、成活率。结果病毒感染组及5-Fu组24h后细胞成活率明显低于空白对照组及阴性对照组，同时病毒感染组细胞出现明显细胞病变。结论C6胶质瘤细胞在体外对黏液瘤病毒易感，黏液瘤病毒在体外对C6胶质瘤细胞有杀灭作用。

Objective To evaluate the susceptibility of C6 glioma cells to Myxoma virus and the killing effect of Myxoma virus to the C6 glioma cells in vitro. Methods C6 glioma cells were infected with myxoma virus, used death virus as the negative control, 5-FU as the positive control, DEMD as blank control. The number of living cells were counted every 24 h, and Western-Blot method, inverted microscope and MTT assay were applicated to observe the cell morphology and survival rate in each group. Results The cell number were decreased rapidly in virus effected group and 5-FU group, with significant differences to the negative and blank control groups. And cells in virus effected group appeared cytopathic effect. Conclusions C6 glioma ceils were susceptible to myxoma virus and myxoma virus had killing effect to C6 glioma cells in vitro.