幼年猪全阴囊Ⅲ度烧伤创面早期植皮修复对睾丸Survivin蛋白表达的影响

EFFECT OF EARLY DERMATOPLASTIC REPAIR ON TESTICULAR Survivin PROTEIN EXPRESSION IN JUVENILE PIGS WITH ENTIRE THIRD DEGREE BURN WOUND OF SCROTUM

目的探讨幼年猪全阴囊Ⅲ度烧伤后早期植皮修复阴囊对睾丸生精功能恢复的影响。方法取2月龄雄性贵州小型猪30只,体重10～15 kg;随机分为3组,每组10只,分别为正常对照组(A组)、自然愈合组(B组)、植皮组(C组)。A组小型猪不作任何处理;B、C组小型猪制备阴囊Ⅲ度烧伤模型后,B组不作处理,待其自行愈合;C组切除烧伤阴囊皮肤,取下腹部全厚皮植皮修复。术后观察B、C组实验动物一般情况,并于模型制备3个月(实验动物已成年)及1年每组分别取5只实验动物双侧睾丸标本,HE染色观察不同方式修复阴囊对睾丸生精细胞的形态学影响,免疫组织化学染色检测Survivin蛋白表达。结果实验动物均存活至实验完成,创面均顺利愈合。组织学观察示A组各级生精细胞形态正常,管腔中可见成熟精子;B组生精上皮明显变薄,仅1～2层生精细胞;C组生精细胞较B组稍多,部分可见精子细胞;术后1年B、C组生精细胞较3个月时增加。免疫组织化学染色示术后3个月及1年B、C组Survivin蛋白表达少于A组,B组少于C组,差异均有统计学意义(P<0.05);组内两时间点比较,差异均无统计学意义(P>0.05)。结论植皮修复全阴囊Ⅲ度烧伤创面对猪睾丸生精功能有抑制作用,可导致生精细胞减少,Survivin蛋白表达降低,但生精小管内仍有部分精子细胞残存。

Objective To explore the effect of early scrotal dermatoplasty on spermatogenic functional rehabilitation of testis in juvenile pigs with third degree burn wound of the scrotum.Methods Thirty healthy male Guizhou miniature pigs（weighing 10-15 kg,2-month-old） were divided into 3 groups： control group（group A,n=10）,natural healing group（group B,n=10）,and dermatoplasty group（group C,n=10）.In group A,the pig was not given any treatment;after third degree burn model of the scrotum was prepared,wounds were not treated in group B and the burn skin was excised and whole hypogastric pachydermia was used for dermatoplasty in group C.At 3 months and 1 year after model preparation,bilateral testis were collected from 5 pigs,respectively.HE staining was performed to observe the effects of different repair method on the morphology of spermatogenic cells and immunohistochemical staining was used to detect Survivin protein expression.Results All pigs survived to the end of the experiment and the wound healed successfully.Histological observation showed that spermatogenic cells had normal shape at all stages and mature sperms were seen in lumens in group A;the thickness of seminiferous epithelium was thinner,having one layer or two layers of spermatogenic cells in group B;the spermatogenic cells in group C were slightly more than that in group B with some spermatids;and in groups B and C,the spermatogenic cells at 1 year were more than that at 3 months.Immunohistochemistry staining showed that the Survivin protein expression in groups B and C was less than in group A,and group B was less than group C,showing significant differences at 3 months and 1 year（P 0.05）,but no significant difference between 3 months and 1 year in the same group（P 0.05）.Conclusion Dermatoplasty has inhibitory effect on spermatogenic functional rehabilitation of testis.Dermatoplasty can decrease spermatogenic cells and reduce Survivin protein expression,but some spermatids still survive in seminiferous tubule.