Runt相关转录因子3 mRNA在口腔鳞状细胞癌中的表达

Expression of RUNX3 mRNA in oral squamous cell carcinoma

目的检测口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)中Runt相关转录因子3(runt-relatedtranscription factor 3,RUNX3)mRNA的表达。方法应用逆转录聚合酶链反应分析临床收集的10例口腔正常黏膜组织和30例OSCC组织中RUNX3 mRNA的表达及其与组织病理分型之间的关系。结果 OSCC组织中RUNX3 mRNA表达水平较正常口腔黏膜组织明显下调。30例OSCC标本中73.3%(22/30)的病例呈现表达下调或缺失,且与肿瘤的分化程度呈负相关(P=0.001)。结论 OSCC中RUNX3 mRNA的表达下调或缺失是一种常发事件。

Objective To detect the mRNA expression of Runt-related transcription factor 3（RUNX3） in oral squamous cell carcinoma（OSCC）.Methods Ten normal oral mucosa and 30 OSCC tissues were collected to examinemRNA expression of RUNX3 by RT-PCR analysis;in addition,the relationship between RUNX3 mRNA expression with the differentiation grades was statistically analyzed.Results In 30 OSCC samples,73.3%（22/30） cases showed reduced or absent expression of RUNX3 mRNA.Statistical analysis revealed that the downregulation of RUNX3 mRNA expression was correlated with the differentiation grades in OSCCs（P=0.001）.Conclusion Downregulation of RUNX3 occurred frequently in OSCC and played an important role in oral carcinogenesis.