药物扑热息痛分子模板聚合物的选择性富集与识别特性研究

Studies of Selective Enrichment and Recognized Characteristics of Paracetamol Molecular Template Polymer

采用分子印迹技术合成了对药物扑热息痛有高选择性的模板聚合物 .通过 Scatchard分析研究了模板聚合物的结合特性 ;研究了模板聚合物的结合动力学 .结果表明 ,以丙烯酰胺为功能单体的模板聚合物比以甲基丙烯酸为功能单体的聚合物具有更高的结合容量 ,且通过氢键作用形成一类等价的结合位点 ,其结合位点的离解常数为 Kd=2 .2 5× 1 0 - 3 mol/L.底物的选择性结合实验表明 ,与组成相同的非模板聚合物相比 。

A molecular templated synthetic polymer as the receptor highly selective for paracetamol was prepared by a molecular imprinting technique. The selective binding characteristics of the template polymer were evaluated by Scatchard analysis and Ultraviolet spectrometry. The results showed that the acrylamide as the functional monomer was more better than methacrylic acid for paracetamol selectivity, and a class of the same binding sites which could specially bind paracetamol by the interaction of hydrogen bonding were formed in the polymer and its dissociation constant was estimated to be 2 25×10 -3 mol/L. The selective binding experiment for substrates indicated that the polymer gave a higher affinity and selectivity for paracetamol. It is possible to be a good adsorption and binding material in the selective enrichment and determination of trace paracetamol in complex biosamples.