驱虫斑鸠菊提取物指纹图谱与其对人A375黑素瘤细胞增殖作用的灰关联度分析

Grey relational analysis on fingerprint of Vernonia anthelmintica extracts and its effect on proliferation of A375 human melanoma cells

目的:建立驱虫斑鸠菊不同乙醇浓度提取物指纹图谱,考察各醇提物共有峰成分对人A375黑素瘤细胞增殖作用的影响;探讨驱虫斑鸠菊醇提物指纹图谱与药效的相关性,为建立驱虫斑鸠菊谱效结合的中药质量控制模式提供理论依据和数据支持。方法:采用HPLC建立驱虫斑鸠菊不同乙醇浓度提取物指纹图谱,并对其进行相似度评价;用四甲基偶氮唑蓝(MTT)比色法研究人A375黑素瘤细胞增殖作用;用灰关联度分析法研究"谱效关系"。结果:驱虫斑鸠菊醇提物指纹图谱有13个共有峰,其对人A375黑素瘤细胞有增殖作用,其中4号峰对其增殖作用贡献最大,灰关联度最高。结论:驱虫斑鸠菊不同乙醇浓度提取物的指纹图谱存在一定差异,指纹图谱共有峰与其对细胞增殖的作用之间有一定的关联性。

Objective： To establish the fingerprint of Vernonia anthelmintica extracts of different ethanol concentrations to study the effect of common peak components on the proliferation of A375 human melanoma cells and the correlation between fingerprint and pharmacodynamics of V.anthelmintica extracts,in order to provide both theoretical basis and data support for establishing a traditional Chinese medicine quality control mode with the combination of fingerprint and pharmacology.Method： HPLC was applied to establish fingerprint of V.anthelmintica extracts of different ethanol concentrations and the similarity evaluation was made.MTT was used to study the proliferation of A375 human melanoma cells.Grey relation analysis was adopted to analyze the correlation between fingerprint and pharmacology of V.anthelmintica.Result： The HPLC fingerprint of different V.anthelmintica ethanol extracts showed 13 common peaks with the proliferation effect on A375 human melanoma cells.The 4th peak had the largest contribution and highest correlation to cell proliferation.Conclusion： Different V.anthelmintica ethanol extracts have different fingerprints,which show a certain correlation between their common peaks and effect on cell proliferation.